1.1 This practice covers and is designed to determine the relative effectiveness of antimicrobial handwashing agents in reducing transient microorganisms using a controlled handwash. 1.2 Knowledge of microbiological techniques is required for these procedures. 1.3 This practice is used to evaluate topical antimicrobial handwashing formulations. 1.4 Performance of this procedure requires the knowledge of regulations pertaining to the protection of human subjects (21 CFR Parts 50 and 56). 1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For more specific precautionary statements, see 8.1. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.